雷公藤红素诱导人肥大细胞系HMC-1细胞凋亡及与细胞周期的关系

Apoptosis of Human Mast Cell-1 Induced by Tripterine and Its Relation to Cell Cycles

目的:研究雷公藤红素对人肥大细胞系 HMC-1细胞凋亡的诱导作用。方法:HMC-1细胞与雷公藤红素(0.125～1.0μmol/L)共育不同时间,经 DNA 琼脂糖电泳和流式细胞仪 DNA 含量分析。结果:(1)雷公藤红素能有效地诱导 HMC-1细胞凋亡,细胞呈凋亡形态学改变,DNA 琼脂糖电泳出现梯形条带,流式细胞仪分析,在 G_1期峰前出现凋亡峰。(2)凋亡程度随药物浓度增加和作用时间的延长而提高。(3)凋亡率随 S 期细胞比例的下降而升高,二者呈显著负相关(P<0.01)。结论:雷公藤红素可以诱导 HMC-1细胞凋亡,凋亡主要发生在 S期。

Objective:To explore the effect of tripterine in inducing human mast cell line(HMC-1)cell apoptosis.Methods: Cells were exposure to tripterine(0.125-1.0 μmol/L)for different time and assayed by DNA electrophoresis and flow- cytometry.Results:(1)Apoptosis of HMC-1 cells could be efficently induced by tripterine with the presence of apoptotic change in morphology.DNA ladder on argrose gel electrophoresis and apoptotic peak before G_1 phase of cell cycle on flow- cytometry;(2)The magnitude of apoptosis increased with the augmentation of tripterine concentration and duration of exposure;(3)With diminution of Sphase cells,apoptotic cells increased.They bore significant negative relation(P<0. 01).Conclusion:The results suggested that tripterine could efficiently induce HMC-1 cell apoptosis,occurring mainly in S phase.