艾灸涌泉穴对老年大鼠自由基代谢影响的研究

A Study on Metabolism of Free Radicals in Senile Rats by Management with Moxibustion on Yongquan(涌泉)Point

目的:探讨艾灸延缓衰老的作用机理。方法:建立自然衰老大鼠模型,分为老年时照组、维生素 E 组及艾灸组,观察大鼠血清和脑组织超氧化物歧化酶(SOD)活性、过氧化脂质(LPO)含量。结果:老年大鼠组血清和脑组织 SOD 活性明显低于青年大鼠组,LPO 含量明显高于青年大鼠组。艾灸组灸涌泉穴后,与老年对照组比较,其血清和脑组织 SOD 活性明显升高(45.30±6.17、35.03±3.67u/ml,P<0.01;2399±316、2005±206u/g 脑组织湿重,P<0.01); LPO 含量明显降低(3.30±0.55、4.40±0.51μmol/L,P<0.01;341±69、446±44nmol/g 脑组织湿重,P<0.01)。维生素 E 组大鼠与老年对照组大鼠比较,其血清和脑组织 SOD 活性差异无显著性。结论:艾灸涌泉穴能增强 SOD 活性,提高老年大鼠清除自由基的能力,改善老年大鼠自由基代谢失调的状况,从而阻止自由基损害机体,保护脑功能,延缓脑衰老,推迟衰老进程。

Objective:To investigate the action mechanism of postponing senility with moxibustion.Methods:The model of natural senile rats was used,and senile rats were divided into three groups at random,namely the control group,vitamin E treated group and moxibustion treated group,and the activity of superoxide dismutase(SOD)and lipid peroxide(LPO) levels in serum and brain tissue of senile rats were determined.Results:In senile group,the activity of SOD in serum and brain tissue reduced markedly,the levels of LPO in serum and brain tissue increased notably when compared with those of young rats.After treating with moxibustion,on “yongquan”(K1)point,compared with the control group,the activity of SOD(10~3 u/ml)in serum (45.30±6.17 vs 35.03±3.67,P<0.01) and brain tissue(2399.0±316.1 u/g.wet.wt vs 2005.0±206.2 u/g.wet.wt,P<0.05)increased notably,the levels of LPO in serum(3.30±0.55 umol/ml vs 4. 40±0.51 umol/ml,P<0.01)and brain tissue(341.0±69.0 nmol/g.wet.wt vs 446.0±44 nmol/g.wet.wt,P<0. 01)reduced markedly.The activity of SOD and the levels of LPO in serum and brain tissue of vitamin E group and those of the control group had no significant difference.Conclusions:Moxibustion on “Yongquan”(K1)point could strengthen the activity of SOD,improve the capability of their eliminating free radicals,rectify the disturbance of metabolism of free radicals in senile rats,prevent free radicals damaging organism,protect the function of brain,and postpone brain senility, delay the process of senility.