溶癌灵悬乳剂肿瘤内注射的抗癌作用实验研究

Experimental Study on Anti-Tumor Effect of Intratumoral Injection with Rongailing(溶癌灵)Together with the Extract of Brucea Javanica Shell

目的:探讨溶癌灵合并鸦胆子种皮毒性部分研制新型肿瘤内注射药物的抗癌效果。方法:经乙醇抽提的鸦胆子种皮水溶性部分,以聚乙烯吡咯烷酮为载体,混合消癌灵制成溶癌灵悬乳剂,并以体外细胞毒杀伤及荷瘤动物肿瘤内注射抑瘤实验观察。结果:细胞毒实验显示该剂对体外培养细胞的杀伤效果与鸦胆子种皮提取液相当,但优于消癌灵及5-Fu;体内抑瘤实验表明在抑瘤作用及伤口愈合等方面,该制剂均优于消癌灵、酒精及5-Fu 组。结论:溶癌灵是一种适于肿瘤局部注射治疗的细胞毒性物质,有一定的开发前景。

Objective:To prepare a new intratumoral injection-Rongailing(RAL) and study its anti-tumor effect.Method: The cytotoxic component of Brucea javanica shell was extracted by ethanol extraction method.Then the component was mixed with polyvinylpyrrolidone (PVP) and Xiaoailing(XAL,a Chinese anti-tumor drug),to prepare a suspending emul- sion RAL.The anti-tumor effects of the RAL were evaluated by cytotoxicity experiment in vitro,and tumor-inhibiting experiments in vivo,which were performed on nude mice model bearing HHC15 hepatoma line.Results:Cytotoxicity ex- periments showed that the cytotoxicity of RAL was equivalent to that of the extract of Brucea javanica shell,but higher than that of XAL and 5-FU.The results of tumor inhibiting experiments showed that intratumoral injection with RAL was more effective than XAL,ethanol and 5-FU in respect of inhibiting tumor growth,minimizing skin lesion and healing of wound.Coheir|on:RAL might be a valuable new drug suitable to intratumoral injection for tumor therapy.