树突状细胞对LPAK抗人肝癌细胞的促进作用

PROMOTING EFFECTS OF DENDRITIC CELLS ON LPAK CELLS KILLING HUMAN HEPATOMA CELLS

目的观察人血树突状细胞（DC）联合LPAK细胞（L）体外抗人肝癌BEL-7402细胞（B）的杀伤效应,并对死亡细胞进行形态学分析.材料和方法LD组为DC＋L＋B,L组为L＋B,二组均采用L：B为5：1和10：1两种放靶比,应用TUNEL法及杀伤细胞检测技术,比较LD和L组的抗肿瘤效应。结果人血DC联合LPAK细胞与单用LPAK细胞的抗肿瘤活性比较,LD组＞L组（P＜0.01）,但二者均能诱导BEL-7402细胞调亡;调亡的肿瘤细胞是TUNEL阳性,在LD组较L组明显增多。结论DC能明显增强LPAK细胞对人肝癌细胞的杀伤活性,但不改变LPAK细胞诱导肿瘤细胞调亡的死亡模式。

Objective To observe the effects of human peripheral blood dendritic cells (DC) on Lymphokine and PHA Activated Killer (LPAK,L) cells Killing human hepatoma cell line BEL-7402 (B) in vitro. The mophology of dead cells was also investigated. Metkods Two kinds of ratios of effect and target (5: 1 and 10: 1 ) were usde in LD group (DC+L+B) and L Group (L+B).Killing cell detectation and TDT mediated X-dUTP nick ending labeling (TUNEL) techniques were adapted. Results The difference in antitumor activity between human blood DC combined with LPAK cells and LPAK cells alone was distinct. LD group＞L group (P＜0. 01) ; and both of them could induce apoptosis of hepatoma cell BEL- 7402. The apoptotic cells of LD group were more than which of L group in number. The apoptotic cells were TUNEL positive in light microscopy. Conclusions Human blood DC could enhance the killing activity of LPAK cells against hepatoma cells, but they could not change the apoptotic death model of hepatoma cells induced by LPAK cells.