摘要
目的我们用卵巢癌SKOV<sub>3</sub>细胞株,对裸鼠进行转瘤、应用核酸杂交技术,对肿瘤组织erbB-2、myc、ras基因的扩增进行检测,分析其扩增对卵巢癌的治疗、预后的影响,为卵巢癌的治疗、预后提供实验依据。方法将24只探鼠注射SKOV<sub>3</sub>（1×107／ml）,传瘤16天。将传它探鼠分为对照组,干扰素治疗组、维甲酸治疗组、干扰素十维甲酸联合治疗组,每组6只裸鼠。治疗4周后,取肿瘤组织.按常规方法提取肿瘤组织DNA,用<sup>3</sup>5S它标记的c-myc、ras、erbB-2探针进行Southern杂交,放射自显影3天洗片。结果经用光密度扫描仪扫描,对照组基因的DNA含量最高,干扰素组和维甲酸组校对照组低,联合应用组基因DNA含量最低。结论本实验应用SKOV<sub>3</sub>细胞,对裸鼠转瘤后,应用干扰素及维甲酸联合诱导治疗,c-myc、ras、erbB-2癌基因的扩增明显下降,肿瘤好转,为临床卵巢癌的诱导分化治疗提供了实验依据、但对其抗肿瘤的确切机理仍需要进一步研究,也需临床治疗的进一步验证。
Objective To detect amplification of related genes of ovarian carcinoma and analyse their amplification influence on treatment and prognosis of ovarian carcinoma, Methed Ovarian carcinoma cell SKOV, line was injected into nude mice to induce carcinoma. Then they were treated with IFN and RA, spot hybridization. Result It was showed that oncogenes erbB-2、c-myc、H-ras were significantly reduced. Conclusion It is indicated that oncogenes amplification is related with prognosis.
出处
《中国肿瘤临床与康复》
1998年第6期29-30,共2页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
原癌基因
卵巢癌
扩增
oncogenes Ovarian carcinoma amplification
