人绒毛膜促性腺激素对T细胞表型亚群的作用

The effect of human chorionic gonadotropin on T cell phenotypic subpopulation

目的:旨在阐明人绒毛膜促性腺激素(hCG)对免疫应答调节作用的部分机理,为理解临床某些疾病的发病机理提供理论参考。方法:分别应用荧光显微镜法和流式细胞仪法,观察了hCG对T细胞表型亚群的作用。并用抗hCG各亚单位的单克隆抗体封闭hCG后作上述试验,分析了hCG分子上与上述作用有关的表位所在。结果:hCG在一定剂量范围内可抑制T细胞表型亚群表达CD3、CD4及CD8分子。hCG作用的阻断试验证明抗hCGβ(10号)及抗完整hCG(17号)单克隆抗体可阻断上述抑制作用,而抗hCGα单克隆抗体(4号)则无此作用。结论:本文证明hCG对T细胞表型亚群有不同程度的抑制作用,而hCG分子上与此作用有关的表位则可能存在于包括β亚单位及完整hCG在内的部分。

Objective: To study the part mechanism of human chorionic gonadotropin (hCG) regulatory effect on immune responses for explaining the immunopathogenic mechanism of some diseases. Method : By using both fluoresent microscope and flow cytometer technique. We studied in vitro the effect of hCG on T cell phenotypic subpopulation. Furthermore . We have also analysed the epitopes on the hCG molecule related to the above effects by anti - hCG monoclonal antibodies (McAbs )blocking assay. Results:The ranged doses of hCG(25 - 200u m/ml)inhibit CD3,CD4and CD8 molecules expression of Tcell phenotypic subset. These inhibition effect can be blocked after the effective ranged dose of hCG were neutralized by anti -hCGβ and anti -intact hCG but not anti - hCGα McAbs. Conclusion: Our results indicate that hCG can inhibit T cell phenotypes expression and the epitopes related to these supressive effects of hCG may present both on the hCGβ subunit and the intact hCG.