幽门螺杆菌感染对胃粘膜上皮细胞凋亡的影响

Effect of Helicobacter Pylori on Gastric Epithelial Cell Apoptosis

为了探讨幽门螺杆菌(HP)相关性胃疾病细胞凋亡这一生物学特性,揭示HP与胃癌的关系及其可能的致癌机制,利用DNA缺口末端标记(DNEL)技术对正常胃粘膜、HP阳性和HP阴性慢性胃炎、萎缩性胃炎、不典型增生及胃癌等活检组织共计130例,进行细胞凋亡状态的研究。结果显示,HP性慢性胃炎和萎缩性胃炎的凋亡指数明显增加(P<0.01),而HP阳性不典型增生和胃癌的凋亡指数降低,从慢性胃炎到胃癌,HP阳性组的凋亡指数明显递减。这提示,从慢性胃炎到胃癌形成的过程中,HP可在前期诱导细胞凋亡,而在后期呈现抑制细胞凋亡的倾向,HP相关性胃疾病可能更具恶变倾向。

To explore the status of apoptosis of Helicobacter pylori associated gastric diseases, and to reveal the relationship between HP and gastric carcinoma as well as the possible carcinogenic mechanism by HP. total 130 gastric mucosal biopsy specimens were studied by using DNA nick end labelling technique. The apoptotic indices (AI) in HP - positive chronic gastritis and atrophic gastritis significantly increased, while AI in HP.....-positive dysplasia and gastric carcinoma decreased. From chronic gastritis to gestric carcinoma, there was a significantly gradual decrease in AI. These suggested lhat from chronic gastritis to gastric carcinoma, HP could induce apoptosis at early stage, but perhaps inhibit apoptosis at later stage. There could be a significant canceration tendency in HP associated gastric diseases.