IGF-I对山羊睾丸间质细胞分泌cAMP和睾酮的影响

The Effects of IGF- I Regulating cAMP and Testosteron Secretion in Cultured Goat Leydig Cells

本实验利用山羊睾丸间质细胞体外培养的方法，研究ＩＧＦ－Ｉ对ｃＡＭＰ和睾酮的影响。结果表明：（１）ＩＧＦ－Ｉ可使间质细胞外ｃＡＭＰ积聚能力显著增强（ Ｐ＜０． ０５～ Ｐ ＜０． ００５）。（２）ＩＧＦ－Ｉ可使间质细胞分泌睾酮的能力显著增加（Ｐ＜０．０５～Ｐ＜０．０１）。（３）ＩＧＦ－Ｉ可刺激山羊间质细胞经 ｈＣＧ诱导睾酮分泌显著增多（ Ｐ＜０． ０５～ Ｐ＜０． ０１）。以上结果与催乳素和白细胞介素－１α这些含氮激素（因子）调节睾酮分泌的机理不同。

Leydig cells from 20 to 60 days'goats were cultured in 24-well tissue culture plates in Medium 199 with or without hCG(0. 1 -3. 0 IU/mL)and/or IGF- I (1- 100 ng/mL). The culture media were changed every 24 or 48 h and saved at - 20℃ until assayed for testosterone and extracellular cAMP. The results showed that IGF- I promoted extracellular cAMP accumulation (P <0. 05 - P <0. 005), enhanced both basal and hCG-induced testosterone secretion(P <0. 05- P <0. 01 ). The effects of IGF- I were in dosedependent manner. It was different from the mechanism of prolactin (PRL) and interleukin-1α (IL-1α) regulation of testosterone secretion.