摘要
An interspecies conserved Plasmodium asparagine rich antigen, designated as ARK26, was isolated by immunoscreening P.falciparum genomic DNA expression library with mouse convalescent anti P.yeolii serum. Partial DNA sequence analysis reveals that ARK26 contains clusters of asparagines and no randomly repeated amino acid sequence motifs are observed. A 65 ×10 3 GST fusion protein is expressed by recombinant plasmid PGEX 5X 1(ARK26) in E.coli C strain ABLE K. Computer programs predict that two asparagine rich regions are among the possible antigenic epitopes of p37 encoded by ARK26. Interestingly, the sequence of ARK26 displays significant similarity to yeast and several other species' mitochondrial genes, and its possible function is discussed.
An interspecies conserved Plasmodium asparagine rich antigen, designated as ARK26, was isolated by immunoscreening P.falciparum genomic DNA expression library with mouse convalescent anti P.yeolii serum. Partial DNA sequence analysis reveals that ARK26 contains clusters of asparagines and no randomly repeated amino acid sequence motifs are observed. A 65 ×10 3 GST fusion protein is expressed by recombinant plasmid PGEX 5X 1(ARK26) in E.coli C strain ABLE K. Computer programs predict that two asparagine rich regions are among the possible antigenic epitopes of p37 encoded by ARK26. Interestingly, the sequence of ARK26 displays significant similarity to yeast and several other species' mitochondrial genes, and its possible function is discussed.
