摘要
Abstract Objective To investigate the relationship between calium calmodulin (CaM) changes and neuronal degeneration in the hippocampus of patients with Alzheimer's disease (AD). Methods The human hippocampal tissues post motem were divided into three groups: the young, the aged, and the AD groups. Each group included 10 patients. The patients with AD were identified through clinical manifestations and argentatine (Bodian) stain. Both the young and the aged groups showed no neurological disorders. Some hippocampal tissues were stained by means of Von Kossa's stain, while others were dyed with fluorescence. The calcium in the hippocampal neurons was observed by the help of a microspectrophotometer. The calmodulin (CaM) activity in the hippocampal neurons was investigated with the help of a microspectrofluorometer. Significant analysis was done with t test of double teams. Department of Neurology, General Hospital of PLA, Beijing 100853, China (Lu WF, Tang HC and Wang LN) Institute of Basic Medical Science, Academy of Military Medical Sciences of PLA, Beijing 100850, China (Fan M, Liu SH and Mi RF) Zhang Pang Hospital, Huchang, Henan Province, China (Dong BM) Results The calcium endocellular deposited in black colour after Von Kossa's stain. Densitometric measurement of the intensity of calcium in the neurons showed that it was higher in the aged or the AD group than in the young group, and that some changes in AD group were more significant compared with controls. Densitometric measurement of the intensity of CaM activity in the neurons was calculated by means of measuring the fluorescence in neurons with a microspectrofluorometer. The values showed that the CaM activity increased with the age, more obviously in patients with AD compared with the controls. Conclusions Deposition of calcium may show a change of the calcium homeostasis in the aging process and the development of AD. Increase in CaM activity may demonstrated that the increased caclium related to functions could coexist with the degenerative process in some neurons. Analytical changes of the CaM activity may be more important in reflecting the function of CaM to AD than the CaM content. In sum, the changes of the calcium homeostasis and the calmodulin activity might play a role in the hippocampal neuronal degenerating process in AD. Further study would be needed for deeper exploration and clarification.
Abstract Objective To investigate the relationship between calium calmodulin (CaM) changes and neuronal degeneration in the hippocampus of patients with Alzheimer's disease (AD). Methods The human hippocampal tissues post motem were divided into three groups: the young, the aged, and the AD groups. Each group included 10 patients. The patients with AD were identified through clinical manifestations and argentatine (Bodian) stain. Both the young and the aged groups showed no neurological disorders. Some hippocampal tissues were stained by means of Von Kossa's stain, while others were dyed with fluorescence. The calcium in the hippocampal neurons was observed by the help of a microspectrophotometer. The calmodulin (CaM) activity in the hippocampal neurons was investigated with the help of a microspectrofluorometer. Significant analysis was done with t test of double teams. Department of Neurology, General Hospital of PLA, Beijing 100853, China (Lu WF, Tang HC and Wang LN) Institute of Basic Medical Science, Academy of Military Medical Sciences of PLA, Beijing 100850, China (Fan M, Liu SH and Mi RF) Zhang Pang Hospital, Huchang, Henan Province, China (Dong BM) Results The calcium endocellular deposited in black colour after Von Kossa's stain. Densitometric measurement of the intensity of calcium in the neurons showed that it was higher in the aged or the AD group than in the young group, and that some changes in AD group were more significant compared with controls. Densitometric measurement of the intensity of CaM activity in the neurons was calculated by means of measuring the fluorescence in neurons with a microspectrofluorometer. The values showed that the CaM activity increased with the age, more obviously in patients with AD compared with the controls. Conclusions Deposition of calcium may show a change of the calcium homeostasis in the aging process and the development of AD. Increase in CaM activity may demonstrated that the increased caclium related to functions could coexist with the degenerative process in some neurons. Analytical changes of the CaM activity may be more important in reflecting the function of CaM to AD than the CaM content. In sum, the changes of the calcium homeostasis and the calmodulin activity might play a role in the hippocampal neuronal degenerating process in AD. Further study would be needed for deeper exploration and clarification.
