摘要
Objective To test anti Sa antibody in different autoimmune connective tissue diseases and analyze the relationship between Sa antibody and clinical manifestations and laboratory tests in rheumatoid arthritis. Method Sa antigen was extracted from human placenta. Anti Sa antibody was tested in 40 normal people and 478 connective tissue disease (CTD) patients using Western Blotting (WB). Results Sa antigen was a protein with molecular weights of 50 kD and 55 kD. Anti Sa antibody was positive in 31.9%(61/191) rheumatoid arthritis (RA), 3.0%(2/67) Sjgren's syndrome (SS), 4.3% (2/46) systemic lupus erythmatosus (SLE) and 0% (0/66) Behcet's disease, 0%(0/60) polymyositis/dermatomyositis (PM/DM), 0%(0/66) other CTD and 0%(0/40) normal controls. Anti Sa antibody was different from other auto antibodies in RA. In rheumatoid arthritis its sensitivity, specificity, positive prediction rate, negative prediction rate were 31.9%, 98.6%, 93.8% and 68.5% respectively. Anti Sa antibody positive patients were significantly different from anti Sa antibody negative patients in morning stiffness, ESR, ANA and X ray grade. Conclusion Anti Sa antibody was a new auto antibody for the diagnosis of RA. Anti Sa antibody positive patients seem to have more serious inflammation and more advanced disease process.
Objective To test anti Sa antibody in different autoimmune connective tissue diseases and analyze the relationship between Sa antibody and clinical manifestations and laboratory tests in rheumatoid arthritis. Method Sa antigen was extracted from human placenta. Anti Sa antibody was tested in 40 normal people and 478 connective tissue disease (CTD) patients using Western Blotting (WB). Results Sa antigen was a protein with molecular weights of 50 kD and 55 kD. Anti Sa antibody was positive in 31.9%(61/191) rheumatoid arthritis (RA), 3.0%(2/67) Sjgren's syndrome (SS), 4.3% (2/46) systemic lupus erythmatosus (SLE) and 0% (0/66) Behcet's disease, 0%(0/60) polymyositis/dermatomyositis (PM/DM), 0%(0/66) other CTD and 0%(0/40) normal controls. Anti Sa antibody was different from other auto antibodies in RA. In rheumatoid arthritis its sensitivity, specificity, positive prediction rate, negative prediction rate were 31.9%, 98.6%, 93.8% and 68.5% respectively. Anti Sa antibody positive patients were significantly different from anti Sa antibody negative patients in morning stiffness, ESR, ANA and X ray grade. Conclusion Anti Sa antibody was a new auto antibody for the diagnosis of RA. Anti Sa antibody positive patients seem to have more serious inflammation and more advanced disease process.
