摘要
Objective To investigate the effect of histamine type Ⅱ (H 2) receptors of the CD + 8 cells from aplastic anemia (AA) patients on the inhibition of hematopoiesis. Methods Employing the modification of the panning method with monoclonal antibody the CD + 8 cells from peri pheral blood mononulcear cells of 17 aplastic anemia patients were obtained. The ribs moved during thoracic operations from 17 patients with non hematologic diseases were as normal bone marrow resource. According to different group 2×10 4/ml CD + 8 cells from AA patients and/or 1.0×10 5 mol/L or 0.5×10 5 mol/L cimetidine alone or in combination were added in the normal CFU GM cultures. Reusults The normal CFU GM from the rib bone marrows was 147.69±24.75/10 5 cells (±s), if 2×10 4/ml CD + 8 cells from AA patients were added in the cultures, the CFU GM formation significantly decreased (89.49±20.49/10 5 cells, P<0.01). If CD + 8 cells from AA patients and 1.0×10 5 mol/L or 0.5×10 5 mol/L cimetidine were in combination added in the cultures, the CFU GM were 144.96±25.34/10 5 cells or 156.28± 32.81/10 5 cells respectively, compared with normal group P>0.05. If 1.0×10 5 mol/L or 0.5×10 5 mol/L cimetidine was added alone in the cultures, the CFU GM number were 110.47±27.46/10 5 cells (compared with control P<0.01) or 160.06±32.22/10 5 cells (compared with control P>0.05) respectively. Conclusion 1.0×10 5 mol/L and 0.5×10 5 mol/L H 2 receptor antagonist cimetidine completely abolished the suppressive effect of CD + 8 cells from AA patients on the growth of normal CFU GM and lower concentration cimetidine (0.5×10 5 mol/L) didn't suppress the growth of normal CFU GM.
Abstract Objective To investigate the effect of histamine type Ⅱ (H 2) receptors of the CD + 8 cells from aplastic anemia (AA) patients on the inhibition of hematopoiesis. Methods Employing the modification of the panning method with monoclonal antibody the CD + 8 cells from peri pheral blood mononulcear cells of 17 aplastic anemia patients were obtained. The ribs moved during thoracic operations from 17 patients with non hematologic diseases were as normal bone marrow resource. According to different group 2×10 4/ml CD + 8 cells from AA patients and/or 1.0×10 5 mol/L or 0.5×10 5 mol/L cimetidine alone or in combination were added in the normal CFU GM cultures. Reusults The normal CFU GM from the rib bone marrows was 147.69±24.75/10 5 cells (±s), if 2×10 4/ml CD + 8 cells from AA patients were added in the cultures, the CFU GM formation significantly decreased (89.49±20.49/10 5 cells, P<0.01). If CD + 8 cells from AA patients and 1.0×10 5 mol/L or 0.5×10 5 mol/L cimetidine were in combination added in the cultures, the CFU GM were 144.96±25.34/10 5 cells or 156.28± 32.81/10 5 cells respectively, compared with normal group P>0.05. If 1.0×10 5 mol/L or 0.5×10 5 mol/L cimetidine was added alone in the cultures, the CFU GM number were 110.47±27.46/10 5 cells (compared with control P<0.01) or 160.06±32.22/10 5 cells (compared with control P>0.05) respectively. Conclusion 1.0×10 5 mol/L and 0.5×10 5 mol/L H 2 receptor antagonist cimetidine completely abolished the suppressive effect of CD + 8 cells from AA patients on the growth of normal CFU GM and lower concentration cimetidine (0.5×10 5 mol/L) didn't suppress the growth of normal CFU GM.
