摘要
Abstract Objective To investigate the effects of wild type p53 (wtp53) on inducing apoptosis by restoring wtp53 expression in pancreatic adenocarcinoma cell line (PC 2) which contains mutant p53, and the interaction between murine double minute 2 (MDM2) and wtp53 in pancreatic adenocarcinoma. Methods A recombinant retroviral vector expressing wild type p53 was constructed and packaged by packaging cell line PA317 cells using calcium phosphate coprecipitation method. The supernatant of the packaged cells PA317 was used to transfect the pancreatic carcinoma cell line (PC 2), then a transformed cell line PC 2/swtp53 was established. The recombinant vector pCMV MDM2 was transduced into PC 2/swtp53 cell line by lipofectin mediated method, a double transfected cell line (PC 2/swtp53/pCMV MDM2) was formed. To determine the integration and expression of exogenous wtp53 gene in the transfected cells, polymerase chain reaction (PCR), Western blot and immunoprecipitation analyses were performed. Apoptosis was analyzed by means of flow cytometry, in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) analysis and DNA agarose gel electrophoresis. Results Transduction with the retroviral vector resulted in integration and expression of wtp53 gene in host cells. The apoptotic cells in PC 2/swtp53 and PC 2/swtp53/pCMV neo cell lines were 12.1%- 12.9%, while the double transfected cell line, PC 2/swtp53/pCMV MDM2, showed less (3.2%) apoptotic cells than its parent cell lines. Conclusions Restoration of expression of wild type p53 with a retroviral vector can increase programmed cell death of pancreatic adenocarcinoma cells (PC 2) containing mutant p53. The overexpression of MDM2 protein has a negative regulating role in wtp53 induced apoptosis in PC 2 cell.
Objective To investigate the effects of wild type p53 (wtp53) on inducing apoptosis by restoring wtp53 expression in pancreatic adenocarcinoma cell line (PC 2) which contains mutant p53, and the interaction between murine double minute 2 (MDM2) and wtp53 in pancreatic adenocarcinoma. Methods A recombinant retroviral vector expressing wild type p53 was constructed and packaged by packaging cell line PA317 cells using calcium phosphate coprecipitation method. The supernatant of the packaged cells PA317 was used to transfect the pancreatic carcinoma cell line (PC 2), then a transformed cell line PC 2/swtp53 was established. The recombinant vector pCMV MDM2 was transduced into PC 2/swtp53 cell line by lipofectin mediated method, a double transfected cell line (PC 2/swtp53/pCMV MDM2) was formed. To determine the integration and expression of exogenous wtp53 gene in the transfected cells, polymerase chain reaction (PCR), Western blot and immunoprecipitation analyses were performed. Apoptosis was analyzed by means of flow cytometry, in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) analysis and DNA agarose gel electrophoresis. Results Transduction with the retroviral vector resulted in integration and expression of wtp53 gene in host cells. The apoptotic cells in PC 2/swtp53 and PC 2/swtp53/pCMV neo cell lines were 12.1%- 12.9%, while the double transfected cell line, PC 2/swtp53/pCMV MDM2, showed less (3.2%) apoptotic cells than its parent cell lines. Conclusions Restoration of expression of wild type p53 with a retroviral vector can increase programmed cell death of pancreatic adenocarcinoma cells (PC 2) containing mutant p53. The overexpression of MDM2 protein has a negative regulating role in wtp53 induced apoptosis in PC 2 cell.
