摘要
Oryza meyeriana Baill is one of the three wild rice species found in China. O.meyeriana possesses valuable characteristics but is reluctant in cell culture in vitro . In a series of experiments, callus with no regeneration ability was induced from young panicle of O.meyeriana. The callus was subcultured and propagated. Embryogenic cell clones were obtained after cryopreservation. Suspension cultures were established and protoplasts were isolated and regenerated into plants. Results of artificial inoculation of Xanthomonas campestris pv. Oryzae showed that the strong resistance did not change in the regenerated plants. The development of protoplast to plant system is an important progress towards utilization of O.meyeriana via cellular engineering. The experiments demonstrated that cryopreservation of plant calli was a new way to obtain embryogenic cell line.
Oryza meyeriana Baill is one of the three wild rice species found in China. O.meyeriana possesses valuable characteristics but is reluctant in cell culture in vitro . In a series of experiments, callus with no regeneration ability was induced from young panicle of O.meyeriana. The callus was subcultured and propagated. Embryogenic cell clones were obtained after cryopreservation. Suspension cultures were established and protoplasts were isolated and regenerated into plants. Results of artificial inoculation of Xanthomonas campestris pv. Oryzae showed that the strong resistance did not change in the regenerated plants. The development of protoplast to plant system is an important progress towards utilization of O.meyeriana via cellular engineering. The experiments demonstrated that cryopreservation of plant calli was a new way to obtain embryogenic cell line.
基金
ProjectpartiallysupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .392 70 4 36 )
FoundationforOutstandingYoungTeachersofChina
StateKeyProgramofChinaandNaturalScienceFoundationofHubeiProvince
China .
