氰戊菊酯特异性人工抗原的合成与鉴定

Synthesis and Identification of Fenvalerate Specific Artificial Antigen

基于农药小分子人工抗原设计合成的基本原则,以氰戊菊酯的特征菊酸部分——氰戊菊酸作为半抗原,与载体蛋白BSA偶联合成了氰戊菊酯免疫特异性人工结合抗原,并对抗原合成条件进行了探索。结果表明,抗原合成反应温度以室温为宜,偶联时缓冲液的最佳pH值为8.0;通过紫外分光光度仪扫描,合成抗原的紫外图谱相对于半抗原和载体蛋白均发生了偏移,表明偶联成功;SDS-PAGE电泳结果表明,合成产物蛋白条带明显在载体蛋白条带之后,即合成产物的分子量大于相应的载体蛋白分子量,从而判定偶联是成功的。

This study has developed a synthesis method for fenvalerate immune antigen based on the synthesis principle of pesticide with small molecular weight. The study utilized the specific acid part of fenvalerate as the hapten which was conjugated to a carrier protein-BSA. The synthesis conditions have also been investigated. Results： The best reaction temperature was at room temperatu^e and the optimum pH for conjugation was at 8.0. The UV-spectrogranl of fenvalerate artificial antigen was different from that of hapten and carrier protein and the electrophoretogram both indicated that the artificial antigen were synthesized successfully.