野生植物刺梨SOD提取液防治大鼠慢性四氯化碳肝损伤

Effect of SOD Extract of Wild Cili on Protection Against Carbon Tetrachloride-induced Rat Liver Damage

目的:观察野生剌梨超氧化物歧化酶(SOD)提取液对大鼠慢性四氯化碳肝损伤的保护和治疗作用。方法:36只雄性Wistar大鼠皮下注射四氯化碳制造慢性肝损伤模型。预防组9只在造模同时给予刺梨SOD提取液灌胃,6周后处死 治疗组9只在造模停止后同样给药6周后处死。相应设立未预防对照组9只和未治疗对照组9只作比较研究。结果:预防组血清ALT明显低于未预防组(P<0.05):治疗组血清AST明显低于未治疗组(P<0.05)。肝组织匀浆脂质过氧化指标中预防组MDA水平明显低于未预防组(P<0.05),GBH-Px水平明显高于未预防组:治疗组GSH-Px水平明显高于未治疗组(P<0.05)。肝组织病理学观察证明预防组HAI积分明显低于未预防组(P<0.05)。结论:口服植物源性野生刺梨SOD制剂作为抗氧化剂对大鼠慢性四氯化碳肝损伤有一定预防保护和治疗作用。

Background/Aims: To investigate the preventive and therapeutic effects of SOD extract of wild Cih on rat chronic liver damage induced by carbon tetrachloride. Methods: The models of thirty-six male Wistar rats with chronic liver damage were estabilished by subcutaneously injecting carbon tetrachlonde. Those were divided into 4 groups, nine rats of preventive group were given SOD extract of wild Cih during modelling and were killed afterwards six weeks. Nine of treated group were given the same medicine for six weeks during modelling and then killed. Nine rats each in unpreventive and untreated were used as controls. Results: The serum levels of ALT in preventive and treated groups were significantly lower than those in unprevent and untreated groups, respectively (P<0.05). As markers of hpid peroxidation, MDA levels of liver tissue in preventive group were significantly lower and GSH-Px were markedly higher than those in unpreventive group (P<0.05). In treated group, GSH-Px levels were also higher than those in untreated group. On histopathologic examination, the HAI Knodell score in preventive group was significantly lower than that of unpreventive group (P<0.05). Conclusions: SOD extract of wild Cili has some preventive and therapeutic effect on chronic liver damage induced by carbon tetrachloride.