急性淋巴细胞白血病bcr/abl融合基因表达及其与免疫表型的关系

Expression of bcr/abl Fusion Gene and Its Relationship with Immunophenotype in Acute Lymphoblastic Leukemia

采用逆转录-聚合酶链反应(RT-PCR)技术检测了M-bcr/abl和m-bcr/abl两种类型融合基因在48例急性淋巴细胞白血病(ALL)患者的表达,并且绝大多数病例同时进行了免疫表型和DNA倍体的流式细胞术分析。结果表明,25%(12/48)的病例M-bcr/abl或m-bcr/abl融合基因阳性,m-bcr/abl阳性率略高。分析bcr/abl阳性病例的免疫表型,发现大多数病例为B系标志阳性,但部分T系标志阳性的病例也检测出M-bcr/abl或m-bcr/abl,说明bcr/abl阳性ALL病例以B系标志阳性者居多,T系标志阳性的ALL病例也可为bcr/abl阳性。

Expression of bcr/abl fusion gene was detected in 48 cases of acute lymphoblastic leukemia (ALL) by reverse transcription-polymerase chain reaction (RT-PCR) and most of the cases were analyzed their immunophenotype and DNA ploidy hy flow cylometry. It was found that 25% of the cases expressed M-bcr/abl or m-bcr/abl fusion gene. More cases were m-bcr/abl fusion gene positive than that of M-bcr/abl. Most bcr/abl-positive samples expressed B-lineage markers. But some samples with T-lineage markers also positive for bcr/abl, even in one case who expressed only pure T markers. Our findings indicate that bcr/abl fusion gene was mainly expressed but not limited in B-lineage ALL, T-lineage ALL also expressed bcr/abl fusion genes.