摘要
本文采用异硫氰酸胍-酚-氯仿一步法成功地提取了日本血吸虫虫卵总RNA,琼脂糖电泳结果呈现18S一条主带,与日本血吸虫成虫RNA的电泳结果一致。日本血吸虫虫卵RNA的抽提成功为虫卵cDNA库的构建以及虫卵有效抗原的筛选奠定了基础。
We have extracted RNA from eggs of Schistosoma japonicum using the single step trophoresis showed a main band of 18s,as similar as RNA from adult worms of Schistosoma japonicum.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
1997年第5期287-288,共2页
Chinese Journal of Schistosomiasis Control