烟曲霉震颤素B复合抗原的制备及鉴定

The preparation and identification of fumitremorgin B-hemisuccinate-carrier proteins

为建立快速、简便、灵敏的检测烟曲霉震颤素Ｂ（ｆｕｍｉｔｒｅｍｏｒｇｉｎＢ，ＦＴＢ）的定量检测方法，利用自行制备的ＦＴＢ制备出３种ＦＴＢ复合抗原。首先将ＦＴＢ的Ｃ６位羟基与琥珀酸酐反应，制备出ＦＴＢ琥珀酸单酯化合物（ＦＴＢ－ｈｅｍｉｓｕｃｃｉｎａｔｅ，ＦＴＢＳ），经元素分析和光谱分析后，再用常规方法与３种载体蛋白脱水结合形成酰胺化合物。对合成路线、中间体的化学结构等进行了分析鉴定。为提高准确性，用红外光谱法代替紫外吸收光谱法判断结合物的生成。

To find the quick, convenient and sensitive method for detecting quantitatively the fumitremorgin B(FTB), three complex antigens were produced. First the hydroxyl group at C-6 position of FTB was reacted with hemisuccinate,forming the FTB-hemisuccinate(FTBS), then the compound was identified by ultimate and spectrometric analysis. The FTBS was combined with three carrier proteins respectively to form the amide compound. The preparating way and chemical structure of intermediate was analysed. Instead of ultraviolet absorption spectrometry, the infrared specteroscopy was used to detecting the complex antigens for the higher accuracy. Author's address Liu Jiang, Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, PRC.