肝硬化大鼠肝脏及血浆内皮素水平的动态变化

Dynamic Detections of Endothelin Concentrations of Liver Tissue and Plasma in Cirrhotic Rats

目的:本研究观察大鼠肝组织及血浆内皮素(ET)水平在肝硬化门脉高压形成过程中的动态变化及其与门脉压力的关系。方法:经皮下注射四氯化碳制作大鼠肝硬化模型,于第0、5、7、9、11周各处死一批动物。以放免法测定肝组织及血浆ET含量,门脉压力用检压计测定。结果:在CCl_1诱导肝硬化过程中,各阶段大鼠肝组织及血浆ET含量均逐渐升高,经t检验有显著性差异(P均<0.01),其中血浆ET增加的幅度不如肝组织大。另外,肝组织ET含量与门脉压力有显著相关性(r=0.612,P<0.01)。结论:本研究结果提示:ET至少部分地通过其在肝内含量升高而增加肝内血管阻力,参与门脉高压的形成。

Aims: To investigate the dynamic changes of liver tissue and plasma endothelin (ET) concentrations in process of cirrhogenesis in rats, and its relationship to portal pressure. Methods: Cirrhosis was induced in rats by percutaneous administration of carbon tetrachloride (1:1 V/ V in cotton seed oil. 3ml/kg). At week 0, 5. 7. 9. 11, animals were killed. Liver tissue and plasma ET concentrations were measured by radioimmunoassay. and portal pressure was determined by manometer. Results: ET levels of liver tissue and plasma increased gradually in process of cirrhogenesis in rats, and with t test there were significant differences (P<0.01 all). In addition, a significant correlation was observed between ET and portal pressure at each stage. Conclusions: ET. at least in part via increasing intrahepatic vascular resistance due to in creasing of its liver tissue concentrations, may be involved in the occurence of portal hypertension in cirrhosis.