摘要
成年SD大鼠分成对照、尾部电流所致伤害性刺激、面部颧髎穴电针三组，分别观察弓状核、中脑中央灰质和延髓头端腹内侧区原位杂交后在神经元中首脑啡肽联原(PPE)mRNA的表达与Fos免疫阳性神经元的分布。痛刺激后上述各部位的PPEmRNA在神经元内均有不同程度表达，但当颧髎穴电针时表达更明显，细胞数也较多。尤其在延髓头端腹内侧区更显著，而在对照动物仅在面神经核等与运动有关核团出现表达，在PPEmRNA与Fos免疫组化相结合实验中．痛组与针刺组动物中皆可观察到一定比例数神经元的共存，及单独Fos和单独EnkmRNA阳性神经元的存在。
Changes of preproenkephalin (PPE) mRNA and Fos in hypothalamus arcuate nucleus (ar) -periaqueductal gray (PAG) - rostral ventro - medial surface of the medulla oblongata (RVM) were studied in SDadult rats which were divided into 3 groups: (l) nociceptive stimulation, (2) electroacupunctrue (EA) and (3)free activity as control. By using methods of in situ hybridization and immunocytochemistry, the PPE mRNA pos-tive neurons, Fos- like immunoreactive (FLI) neurons and PPE mRNA with FLI neurons were found in the rat ar- PAG - RVM. The expression was enhanced by noxious stimulation as shown by the greater number of neuronsand the heavier color staining. In EA group, the expression was increased dramatically, particular in RVM ascompared with that in the nociceptive tail stimulation rats. The results showed that the expression of PPE mRNAwas enhanced after nociceptive and/or EA stimulation, and the coexistence of PPE mRNA with FLI suggests thepossible modulation of PPE mRNA expression by c - fos.
