大鼠鼻粘膜肽能神经末梢分布的研究

A STUDY OF THE DISTRIBUTION OF PEPTIDERGIC TERMINALS IN RAT NASAL MUCOSA

用免疫组化技术（ＡＢＣ法）系统研究了大鼠鼻粘膜９种肽能神经末梢分布的特征，这９种神经肽分别是Ｐ物质（ｓｕｂｓｔａｎｃｅＰ，ＳＰ），神经激肽Ａ（ｎｅｕｒｏｋｉｎｉｎＡ，ＮＫＡ），神经激肽Ｂ（ｎｅｕｒｏｋｉｎｉｎＢ，ＮＫＢ），降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅ－ｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ），血管活性肠多肽（ｖａｓｏａｃｔｉｖｅｉｎｔｅｓｔｉｎａｌｐｏｌｙｐｅｐｔｉｄｅ，ＶＩＰ），神经肽Ｙ（ｎｅｕｒｏｐｅｐｔｉｄｅＹ，ＮＰＹ），甘丙肽（ｇａｌａｎｉｎ，ＧＡＬ），生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ，ＳＯＭ）及神经降压素（ｎｅｕｒｏｔｅｎｓｉｎ，ＮＴ），同时选择与鼻粘膜神经肽（ＮＰ）作用密切相关的三叉神经节（ＴＧ）细胞进行上述ＮＰ的定位。用重组ＰＳＰ６５质粒（４００ＳＯＭｃＤＮＡ）制备ＳＯＭｍＲＮＡ单链探针，以地高辛精标记，在鼻粘膜及ＴＧ细胞进行ＳＯＭｍＲＮＡ的原位杂交组化研究。结果提示大鼠鼻粘膜有丰富的肽能神经末梢；ＴＧ细胞含有多种ＮＰ并且可以合成ＳＯＭ。该研究结果对重新认识鼻粘膜神经分布规律有一定意义。

Both the distributions of nine peptidergic terminals including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), calcitonin gene--related peptide (CGRP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), galanin (GAL), somatostatin(SOM) and neurotesin (NT) in nasal mucosa and localizations of all these neuropeptides(NP) in neurons of trigemenal ganglion (TG) in rat were studied with immunohistochemicaltechnique (ABC method). In addition, SOM mRNA in situ hybridization histochersistry wascarried out in both nasal mucosa and TG cells, with in vitre recombinant PSP65 plasmid(400 SOM cDNA) as a plate for the preparation of SOMmRNA single strand (cRNA)probe, which was labelled with digoxigenin. The results indicated that there were a lot ofpeptidergic terminals in rat nasal mucosa and TG cells might synthesize multiple NPs andthat NPs in nasal mucosa were chiefly released in the nerve terminals. This study helps tooffer a new understanding of the innervation of nasal tnucosa.