P21过表达和P53蛋白蓄积在判断肺癌恶性度与预后的应用研究

PROGNOSTIC SIGNIFICANCE OF THE OVER-EXPRESSION OF ras-P21 AND ACCUMULATION OF P53 PROTEIN IN LUNG CANCER

应用Ha-ras P21和P53单克隆抗体,采用S-P免疫组织化学方法对86例原发性肺癌进行了研究,前者总阳性率为79.07%,后者为53.48%.细支气管肺泡癌的P21强阳性(++)率明显高于腺泡状腺癌和大细胞癌(P<0.05),分化程度越高,染色越强.阴性(-)和中等阳性(+)的5年存活率明显高于强阳性(++)者.P53蛋白蓄积与肺癌的分型、分化、TNM分期无关,但P53阳性与阴性患者的术后平均存活月数间有着显著的差异(P<0.05),P53染色越强则术后存活月数越短.提示P53蛋白蓄积是判定肺癌预后的指标之一.

Using anti-P21 and P53 (DO-1) monoclonal antibodies, the expression of Ha-ras and accumulation of P53 proteins were investigated by streptavidin peroxidase conjugataed method with in paraffin-embeded specimens of 86 primary lung carcinomas. The total positive rate was 79.07% for P21 and 53.48% for P53. The P21 strong positive (++) rate of alveolar bronchiole carcinoma was higher than acinar adenocarcinoma and large cell carcinoma (P<0. 05). The higher the degree of histologic differentiation, the stronger the staining of P21 was. The patients with (-) or middle-positive (+) Ha-ras P21 expression had significantly longer survival time than those with stong positive (H) (P<0. 01). Accumulation of P53 protein was not related to type differentiation and stage of TNM of lung cancer. But there was obvious correlation to the survival time after operation between patients of P53 positive and or negative (P<0. 05). The higher P53 positive count, the shorter the survival time. Therefore, accumulation of P53 protein was one of useful marker to judage the prognosis of primary lung cancer.