马桑内酯对培养的海马神经元γ-氨基丁酸和谷氨酸的影响——免疫细胞化学研究

EFFECT OF CORIARIA LACTONE ON γ-AMINOBUTYRIC ACID AND GLUTAMATE IN THE GULTURED NEURONS OF RAT HIPPOCAMPUS --AN IMMUNOCYTOCHEMICAL STUDY

用免疫细胞化学方法,观察研究了马桑内酯(CL)对培养的海马神经元内γ-氨基丁酸(GABA)和谷氨酸(Glu)神经元的影响.结果表明:CL作用后,GABA免疫反应阳性神经元数目减少,反应强度减弱;Glu免疫反应阳性神经元数目变化不明显,但反应增强.推测:CL可能引起海马神经元兴奋性增高是使动物模型致痫的基础,其机理可能与阻断GABA的合成途径有关.

The effect of coriaria lactone (CL) on Y-aminobutyric acid (GABA) and glutamate (Glu) was studied in the cultured neurons of rat hippocampus by using immunocytochemical method.The results showed that after administrating CL the number of GABA-IR positive neurons decreased and the immunoreactive intensity was lowered, however the number of Glu-IR positive neurons did not reveal any significant change but the immunoreactive intensity was higher. Our findings suggested that CL could enhance excitability in the hippocampus and the increased excitability could be the underlying basis of epilepsy caused by CL in animal models. This mechanfsm may have something to do with the blockade of GABA synthesis process.