四氯化碳中毒性肝硬化肝胶原纤维的定量分析

A QUANTITATIVE ANALYSIS ON THE COLLAGENOUS FIBER IN LIVER WITH TOXIC CIRRHOSIS INDUCED BY CARBON TETRACHLORIDE

本文应用彩色图像分析技术,对四氯化碳中毒性肝硬化胶原纤维含量进行了定量分析.正常鼠肝胶原纤维的体密度为0.09±0.03,注射四氯化碳后45天和60天鼠肝胶原纤维增多,体密度分别为0.52±0.06和3.39±1.68.说明了肝胶原纤维的体密度随肝组织受四氯化碳中毒程度的加深而增加.肝胶原纤维增生是许多慢性肝脏疾病发展为肝硬化的主要病理基础.对于胶原纤维增生程度多凭主观观察,或通过羟脯氨酸含量测定进行间接分析.本文应用先进的彩色图像分析技术对慢性四氯化碳中毒肝胶原纤维增生情况进行了定量分析.材料和方法:Wistar大白鼠30只,分为二组.第一组为实验组20只大白鼠,每周腹腔注射25%四氯化碳石腊油一次0.5ml,动物分两批,每批10只,分别于注射后45天和60天处死.

The collagenous fiber in liver with toxic cirrhosis induced by carbon tetrachloride was quantitatively studied by color image analysis technique. The volume density of collagenous fiber in liver of normal mouse was 0. 09±0. 03, it increased to 0. 53±0. 06 and 3. 39±1. 68, at the 45th and the 60th day after injecting carbon tetrachloride, respectively. It was indicated that the volume density of collagenous fiber increased with the injury degree of liver by carbon tetrachloride.