r－干扰素对喉癌细胞系HEP－2HLA－DR抗原和增殖细胞核抗原表达的影响

EFFECTS OF IFN-r ON HLA-DR ANTIGEN AND PROLIFERATIVE CELLULAR NUCLEUS ANTIGEN (PCNA) EXPRESSION OF HUMAN LARYNGED CANCER CELL LINE (HEP-2)

本实验用人重组ｒ－干扰素（ｒｈｕ－ＩＦＮ）作用ＨＥＰ－２细胞后ＨＬＡ－ＤＲ抗原和增殖细胞核抗原（ＰＣＮＡ）表达的检测来探讨ｒ－干扰素对ＨＥＰ－２细胞ＨＬＡ－ＤＲ抗原表达诱导作用及体外抗增殖活性。用单克隆抗体ＣＲ３／４３（抗ＨＬＡ－ＤＲ）和Ｋｉ－６７（抗ＰＣＮＡ）。以链霉素一生物素技术（ＬＳＡＢ）检测ＨＥＰ－２细胞ＨＬＡ－ＤＲ抗原和ＰＣＮＡ表达，结果显示：ｒ－ＩＥＮ诱导ＨＬＡ－ＤＲ抗原和抑制ＰＣＮＡ表达其强弱与ｒ－ＩＦＮ剂量有关。资料提示：ｒ－ＩＦＮ不仅对ＨＥＰ－２细胞有细胞毒作用，同时能调节其细胞膜特性，因而在喉癌的治疗中是有效的。

The effects of IFN-r on HLA-DR and proliferative cellular nucleus antigen expression ofHEP-2 cell line were investigated using LSAB (labelled sptraptaridin biotin method) withmonclonal antibody CR3/43 (anti-HLA-DR) and Ki-67 (anti-PCNA). The results showedthat HLA-DR antigen which induced and PCNA expression which is inhibited by IFN-r inHEP-2 line, and were dependent on dose. The data suggest that the IFN-r may be useful inthe treatment of larryngeal carcinotna because it provides effective cytotoxicty and cell membrance mudlating properties to HEP-2.