一种冰冻切片漂片法免疫反应后再用石蜡切片的方法

A METHOD OF PARAFFIN￣EMBEDDING SECTIONS FOLLOWING FLOATING-FROZEN SECLION IMMUNOSTAINING

本文采用组织冰冻切片５０μｍ进行双重标记的免疫组织化学漂片染色后，将其贴在塑料包埋盒内，脱水，经石蜡包埋切为１－３μｍ的薄片贴于载玻片上，干燥后脱水透明封片，最后进行切片观察，免疫阳性产物被准确定位于所观察的细胞或纤维上，得到满意的结果。为免疫组织化学形态学研究工作提供了新的手段。

Abstract pm frozen sections, after treated by double immunohistochemical staining, are set onthe bottom of embedding box, dehydrated, cut at 1 - 3μm, embedded in paraffin. Then thesections are dried, dehydrated, cleared, and sealed. Under observation the immuno-positivematerial of these sections was located exactly over the cells or fibres. The result is very sat Keyworks: Immunocytochemistry; Doubleimmunostaining; Parafin embedding; Thin section