丝裂霉素对兔眼睫状体上皮碳酸酐酶及ATP酶活性的影响

The Effect of Mitomycin C on Activities of Carbonic Anhydrase and ATPase of the Ciliary Body Epithelium in Rabbit

目的:采用酶组织化学方法,观察兔眼巩膜咬切术中应用丝裂霉素(MMC)后,MMC对睫状体上皮细胞分泌房水的功能酶-碳酸酐酶(CA)及ATP酶(ATPase)的影响,以期进一步探讨术后MMC降低眼压的作用机制。方法:在兔眼巩膜咬切术中局部应用0.2mg/ml MMC 0.1ml,术后第7天,分别采用Hasson氏法和硝酸铅法对睫状体上皮细胞的碳酸酐酶及ATP酶进行显色,经光镜观察,并采用计算机图像分析仪对酶的灰度值进行分析。结果:MMC对术区附近睫状体上皮细胞的CA及ATPase活性有抑制作用,而远离术区90°部位及生理盐水对照组酶活性改变不明显。结论:MMC可能通过抑制影响房水分泌的CA及ATPase的活性而减少房水分泌,降低眼压。抑制程度大则可能导致持续性低眼压。眼科学报 1996;12:75—78。

Purpose: Enzymohistochemical methods were performed to observe the effect of mitomycin C(MMC) on the activities of carbonic anhydrase(CA)and ATPase of the ciliary body epithelium in rabbits after using 0. 2mg/ml mitomycin C during sclerectomy for the further study on the mechanism of the decrease of intraocular pressure postoperatively. Methods :0. 2mg/ml mitomycin C was topically applicated during the sclerectomy in rabbit eyes. 7 days postoperatively, Hasson's method and plumbum nitrate's method were used to stain the CA and ATPase of the ciliary body epithelium. The grey value of the enzymes was quantitated by Image Analysor. Rusults: MMC inhibited the activities of CA and ATPase of the ciliary body epithelium underlying the site of MMC application, but the difference of enzymatic activities of the region 90 degree apart from the surgical site and that of surgical site in normal saline control group was not statistically obvious (P<0. 05). Conclusions:MMC could inhibit the activities of CA and ATPase of ciliary body epithelium, thus leading to the decreased secretion of humor aqueous and the lowerdown of intraocular pressure even persistent hypotony. Eye Science 1996; 12: 75- 78.