摘要
目的:利用我室建立的视网膜母细胞瘤细胞株SO-Rb_(50)和SO-Rb_(70)探索MTT法用于悬浮生长的RB细胞的有关因素,及骆驼蓬总碱对这两个瘤细胞株体外抑制作用。方法:分别测试瘤细胞数与OD值(光密度值)的关系,MTT作用时间与OD值的关系,及结晶物经DMSO(二甲基亚砜)溶解后的稳定时间,并用MTT法检测了骆驼蓬总碱对这两个瘤细胞株体外抑制效果。结果:这两个瘤细胞株活细胞数量与OD值成正比,MTT作用时间在12小时内OD值渐增加,结晶物经DMSO溶解后的稳定时间为11小时。骆驼蓬总碱作用于SO-Rb_(50)瘤细胞48和72小时的IC50值(μg/ml)分别为10.66和4.82;作用于SO-Rb_(70)瘤细胞48和72小时的IC50值(μg/ml)分别为6.37和4.2。结论:MTT法可用于悬浮生长的Rb细胞,骆驼蓬总碱对SO-Rb_(50)和SO-Rb_(70)瘤细胞有明显的抑制作用。眼科学报 1996;12:83—87。
Purpose :MTT assay was evaluated on cytoxicity for suspension growing cell lines of SO-Rb50 and SO-Rb70,and the toxicity of TAH (the total alkaloid of peqanum harmala L) on the above cell lines was assessed.
Methods: The relationships between cell number and optical density, between optical density and exposure time of MTT,and the stability of formazan crystal solution in MDSO were determined. And the toxicity of TAH on the cell lines of SO-Rb50 and SO-Rb70 in vitro with MTT assay was assessed.
Results: There was a direct proportional relationship between the amount of cell number and its optical density; The optical density increased gradully within 12 hours of the MTT incubation time;The stable time of the formazan crystal solved in DMSO was 11 hours. The IC50 values (μg/ml) of TAH on SO-Rb50 were 10. 66, 4. 82 respectively for 48 and 72 hours;and on SO-Rb70 were 6. 38,4. 2 respectively for 48 and 72 hours.
Conclution: MTT assay can be used for suspension growing cell lines of SO-Rb50 and SO-Rb70:TAH has obvious toxicity to these two cell lines. Eye Science 1996; 12: 83- 87.
基金
卫生部科学基金资助课题的一部分
