α－淀粉酶水解液发酵生产麦角隐亭的研究

Studies on Ergocryptine Production in a Medium Containing 5% Wheat Flour α-amylase Hydrolyzate

用５％麦粉的α－淀粉酶水解液培养麦角菌ＡＴＣＣ２００１９，结果其发酵液能产生麦角隐亭（５０．１６±２．４０）μｇ／ｍＬ，与３０％蔗糖的发酵培养基的产碱量（２０．７４±３．５３）μｇ／ｍＬ相比，两者有差异（ｐ＜０．０５）．在１０％蔗糖的培养基中，发酵第４天后补加５％麦粉的α－淀粉酶水解液，麦角隐亭的产量为（４７．１２±６．３４）μｇ／ｍＬ，也高于碳源为３０％蔗糖的产碱量（１９．８９±２．４０）μｇ／ｍＬ，两者差异显著，ｐ＜０．０１．而两种方法的菌丝体生长量均相差无几，ｐ＞０．０５．

Claviceps purpurea ATCC 20019 could produce ergocryptine 50. 16±2. 40μg/mL in the fermentative medium containing α-hydrolyzate of 5% wheat flour, but the alkaioid yield was 20.74±3. 53μg/mL in the fermentative medium containing 30% sucrose. There was difference between the two, p<0. 05. After 4 days fermentation in medium containing 10% sucrose,hydrolyzate of 5% wheat flour was supplemented. The yield of ergocryptine 47.12±6. 34μg/mLwas higher than that of 30% sucrose 19. 89±2. 40μg/mL, p<0. 01. There was no obvious difference between the mycelia biomasses of the two methods, p>0. 05.