唐菖蒲花序再生体系的建立

Establishment of a regeneration system of gladiolus inflorescence

【目的】为唐菖蒲的遗传转化研究建立高效快速的再生体系。【方法】以4-6叶期唐菖蒲花序的不同部位（花茎、花柄、花托、雌蕊、雄蕊、花瓣）为外植体,以附加不同质量浓度6-BA和NAA的MS为诱导、增殖和继代培养基,探讨唐菖蒲愈伤组织的诱导及植株再生情况。【结果】所用外植体中,雌雄蕊分化效果不明显,花瓣基本无分化,而花茎、花柄、花托的切块接种在MS＋1 mg/L 6-BA＋1 mg/L NAA培养基上,可直接诱导愈伤组织和丛生芽的生成。丛生芽继代增殖在MS＋0.5 mg/L 6-BA＋0.1 mg/L NAA培养基上,增殖效果最好;生根培养时以MS＋0.1mg/L NAA的培养效果最佳。【结论】用幼嫩花茎作外植体可高效地建立起唐菖蒲的再生体系。

[Objective] The aim was to establish an efficient regeneration system for the genetic transformation of gladiolus. [Method] The different parts （floral axis, pedicel, floral receptacle, pistil, stamen, petal） of gladiolus＇s inflorescence which was at 4--6-leaf stage were used as the explant and the MS medium added different ratios of density hormone 6-BA and NAA was regarded as induction, proliferation and subculture medium. The callus induction and plant regeneration of gladiolus were studied. [Result] The result indicated that the differentiation of bisexual pistil was not obvious and the petal was not differentiated basically,but the pieces of the floral axis,the pedicel and the floral receptacle inoculated in the medium of MS＋ 1 mg/L 6-BA＋ 1 mg/L NAA could induce callus and multiple shoots directly. The proliferative effect of multiple shoots＂ subculture cultivated in the medium of MS＋0. 5 mg/L 6-BA＋0. 1 mg/L NAA was the best. The optimum medium for rooting was composed of MS＋0.1 mg/L NAA. [Conclusion] A high frequency regeneration system could be established with gladiolus tender scape.