生防放线菌153活菌剂填料的选择及其防病促生作用研究

Formulation of biocontrol actinomyces strain 153 and its control effect on Verticillium wilt

【目的】筛选153活体菌剂的理想填料,以延长153制剂的货架期,提高其大田防病效果。【方法】以固体发酵培养153,分别添加硅藻土、高岭土和非耕作层黄土3种填料,通过活菌量测定和温室防病促生效果观察对理想填料进行筛选。【结果】在3种填料中,以加入非耕作层黄土的活体粉剂的存储时间最长,35℃下可以保存70 d以上,活菌量保持水平最高。菌株153对茄子的生长发育有明显促进作用,对植株株高、倒3叶叶面积、干质量及根生长的促进作用最为明显,植株鲜质量的增幅最高可达98.47%,地上部干质量和根干质量的增幅最高分别可达74.86%和82.64%。以非耕作层黄土为填料的菌剂对茄子黄萎病的防效可达82.44%。【结论】通过加入合适的填料,能有效延长菌剂贮存期并提高菌株的生态适应性,为生防菌153从实验室走向生产实践奠定了基础。

[Objective] The study was done to prolong the shelf life of actinomycetes strain 153 and improve the bio-control effect in the field grown condition. [Method] 153 solid-state fermented culture was mixed with diatomite,kaolin as well as non-cultivated loess and screened by the number of living bacteria and their protection effect on eggplant against Verticillium dahliae was studied. [Result] Non-cultivated loess was better than diatomite,kaolin. With this additive,the biocontrol agent could be stored more than 10 weeks at 35 ℃. The growth ahd development of eggplant could be promoted obviously by live-cell formulation of 153. Especially,the amplitude of green plant weight attained 98.47% ,amplitude of dry weight of stem and dry weight of radix attained 74.86% and 82.64%. The control rate of eggplant Verticillium wilt with the formulation mixed non-cultivated loess reached 82.44 %. [Conclusion] By adding suitable additive, the formulation of aetinomyces strain 153 can obtain longer shelf life and better environment adaptation.