摘要
Electropolymerized o-phenylenediamine firm is used as a functional coating for the immobilization of anti-S. aureus antibody on the surface of a gold-plated piezoelectric crystal, and this piezoelectric immunosensor is applied to detect S. aureus. The frequency shift (Delta F = F-20s - F-380s, Hz) between the frequency at the 20th second (after the addition of sample, F-20s) and that at 380 seconds later (F-380s) was introduced to construct a calibration graph, and shortening of assay time was achieved. The S. aureus concentrations in the range of 10(5)-10(9) cells/mL can be detected by this system.
Electropolymerized o-phenylenediamine firm is used as a functional coating for the immobilization of anti-S. aureus antibody on the surface of a gold-plated piezoelectric crystal, and this piezoelectric immunosensor is applied to detect S. aureus. The frequency shift (Delta F = F-20s - F-380s, Hz) between the frequency at the 20th second (after the addition of sample, F-20s) and that at 380 seconds later (F-380s) was introduced to construct a calibration graph, and shortening of assay time was achieved. The S. aureus concentrations in the range of 10(5)-10(9) cells/mL can be detected by this system.
基金
Project supported by the National Natural Science Foundation of China and the State Education commission of China.
