一株电泳型独特的猪B组轮状病毒的分离鉴定

Isolation and Identification of a Strain of Group B Rotavirus with Specific Electrophoretype

１９８９年春，在新疆某猪场采集的仔猪腹泻粪样中，以ＰＡＧＥ法检出一株电泳型独特的非典型轮状病毒，其电泳型与Ｃｈａｓｅｙ等［１］报道的猪Ｅ组轮状病毒相似，该毒株样品口服接种剥夺初乳仔猪可在１２～１６ｈ后引起仔猪急性水样腹泻，并可在剥夺初乳仔猪体内稳定传代，但在用ＥＬＩＳＡ和ＣＩＥ法检测时发现该毒株不具有Ａ组轮状病毒组抗原，而具有Ｂ组轮状病毒组抗原．后用Ａ、Ｂ组轮状病毒全基因核酸探针斑点杂交检测，它与Ａ组探针无杂交信号，而与Ｂ组探针则有杂交信号。证明该毒株为一电泳型独特的仔猪Ｂ组轮状病毒．

In detection piglet stool samples which collected ftom a swine herd by PAGE,a specific electgrophoretype of atypical rotavirus was found in 1989.The electrophoretype of the atypical rofavirus was similar to group E rotavirus which reported by Chasey et al.Pigalet orally inoculated with the atypical rotavirus developed acute watery diarrhea during 12～16 hours postinoculation,and the virus can propagated in piglet steadily.By means of ELISA and CIE group-antigen examination and nucleic acid probe hybridization detection it was shown that the virus strain contained group B-antigen but did not contain group A-antigen,it was also shown that the virus strain hybriyized only with group B rotavirus nucleic acid probe but did not hybridize with group A rotavirus nucleiC acid probe.All of these indicated that the virus strain belongs to piglet group B rotavirus which contained a specific electrophoretype.