摘要
he effects of various concentrations of copper,manganese and ph on the growth,protein content and several enzyme activities of mycorrhizal fungus Cenococcum geophilum Fr. Were investigated.The results showed that high concentration of copper (20 mg/L)and low ph (3.0-4.0) inhibited the mycelial growth (dry weight), however, the protein content increased or not significantly reduced in mycelia after copper or manganese was applied. The activities of acid phosphatase and α-mannosidase were induced by high concentration of Mn (200-400 mg/L);but the activities of G6PDH, MTLDH and trehalase were inhibited by high content of copper(10-20 mg/L),both of the responses of these enzymes and mycelial growth under the presence of copper were correlative each other. Measurement of these enzyme activities might therefore provide a useful criterion for the evaluation of the fungitoxicity of soil contaminated by copper.
he effects of various concentrations of copper,manganese and ph on the growth,protein content and several enzyme activities of mycorrhizal fungus Cenococcum geophilum Fr. Were investigated.The results showed that high concentration of copper (20 mg/L)and low ph (3.0-4.0) inhibited the mycelial growth (dry weight), however, the protein content increased or not significantly reduced in mycelia after copper or manganese was applied. The activities of acid phosphatase and α-mannosidase were induced by high concentration of Mn (200-400 mg/L);but the activities of G6PDH, MTLDH and trehalase were inhibited by high content of copper(10-20 mg/L),both of the responses of these enzymes and mycelial growth under the presence of copper were correlative each other. Measurement of these enzyme activities might therefore provide a useful criterion for the evaluation of the fungitoxicity of soil contaminated by copper.
