摘要
R-phycoerythrin from Porphyra haitanensis exists in two aggregaion states with different molecularweights. A more highly aggregated form, RPE I,Was chromatographed on Bio-Rex 70 column with ureasolution （pH 3.0） as eluent, and the molecular weights of the 3 subunits （α,β,γ） obtained were determinedon SDS-PAGE at 18000, 19200 and 30000, respatively. α subunit carried two phycoerythrobilin （PEB）,β subunit, three PEB and one phycourobilin （PUB）,γ subunit, one PEB and three PUB chromophores.The molar ratio of α,β, and γ subunits of RPE I wn 6:6:1, and their subunit composition was con-fired to be （αβ）<sub>6</sub>γon account of the molecular weight of RPE I, 232000.A lower aggregated form, RPE Ⅱ, contained α and β subunits similar to those of RPE I, but itssubunit composition was the （αβ） monomer of RPE.
R-phycoerythrin from Porphyra haitanensis exists in two aggregation states with different molecular weights. A more highly aggregated form, RPE Ⅰ, was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α,β,γ ) obtained were determined on SDS-PAGE at 18000, 19200 and 30000, respectively, α subunit carried two phycoerythrobilin (PEB);βsubunit, three PEB and one phycourobilin (PUB);γ subunit, one PEB and three PUB chromophores. The molar ratio of α,β, and γsubunits of RPE Ⅰ was 6: 6: 1, and their subunit composition was con-fired to be (αβ)6γ on account of the molecular weight of RPEⅠ, 232000.A lower aggregated form, RPE Ⅱ, contained α and β subunits similar to those of RPE Ⅰ, but its subunit composition was the (αβ) monomer of RPE.
