摘要
ＥｘｐｒｅｓｓｉｏｎｏｆＧＳＴ－ＩＬ－１ｆｕｓｉｏｎｇｅｎｅＣｈｅｎＭｅｉｈｏｎｇ（陈梅红）；ＷａｎｇＺｉｌｉｎｇ（王字玲）；ＤｅｎｇＪｉａｎｂｅｉ（邓健蓓）；ＺｈａｏＺｈｏｎｇｌｉａｎｇ（赵忠良）；ＣｈｅｎＮａｎｃｈｕｎ（陈南春）；ＳｕＣｈｅｎｇｚ...
Two GST-IL-1 fusion genes were constructed by inserting different cDNA fragments of human interleukin1 (IL-1) into the 3'-terminus of GST gene in the fusion protein expression vector pGEX-4T. After IPTG induction ,SDS-PAGE was employed to detect the gene expression. No corresponding protein encoded by GST gene fused with the whole-length 816 bp IL-1 cDNA was observed, nor was free GST protein. However, the fusion protein of GST and IL-1 cDNA without the 189 bp at the 5'- terminus was detected, amounting to 30% of the total bacterial protein expressed. This might suggest that the sequence of 1-189 bp of IL-1 cDNA affected the expression of the fusion gene. That is to say, the downstream sequence distant from the translation start codon AUG in the target gene could significantly affect the expression of the fusion gene.
