摘要
ＥｘｐｅｒｉｍｅｎｔａｌｓｔｕｄｉｅｓｏｎｍｙｏｃａｒｄｉａｌｐｒｏｔｅｃｔｉｏｎｗｉｔｈｃｏｎｔｉｎｕｏｕｓｗａｒｍｂｌｏｏｄｃａｒｄｉａｐｌｅｇｉａＣｈｅｎＬｏｎｇ（陈龙）；ＨｕＳｈｏｕｐｅｎｇ（胡寿鹏）；ＺｈａｎｇＢａｏｒｅｎ（张宝仁）；Ｚｈｕ...
Using the cat cardiopulmonary bypass, the present study was designed to ascertain whether continuous warm blood cardiaplegia can prevent heart arrest from myocardial ischemia and reperfusion injury. Forty -two cats weighing 3-3. 5 kg were divided at random into 7 groups. The hearts were not subjected to ischemia/reperfusion in group 1, whereas subjected to 180 min arrest with different myocardial protective methods in group Ⅱ-Ⅳ (namely,with intermittent cold blood cardiaplegia in group Ⅱ, with continuous cold blood cardiaplegia in group Ⅲ, and with continuous warm blood cardiaplegia in group Ⅳ), and subjected to 60 min reperfusion after 180 min arrest on the basis of group Ⅱ-Ⅳ in groups Ⅴ-Ⅶ. One hundred and eighty minutes after cardiac arrest ,mitochondrial function was slightly depressed in group Ⅲ , significantly depressed in group Ⅱ , and normal in group Ⅳ. Mitochondrial function was markedly depressed in group Ⅴ-Ⅶ 180 min after arrest and 60 min after reperfusion , but remained almost normal in group Ⅶ. The levels of ATP content decreased 180 min after arrest in group ⅡⅡ-Ⅲ,but in group Ⅲ myocardial ATP content was maitained at a higher level than in group Ⅲ. In group Ⅳ myocardial ATP content remained normal after 180 min after arrest. MDA content slightly increased in group Ⅲ,significantly increased in group Ⅱ. After reperfusion MDA content rose to a higher level in group Ⅴ-Ⅶ. But MDA content was almost normal in the hearts protected with continuous warm blood cardiaplegia. Our data indicated that the method of continuous warm blood cardiaplegia is safe and effective and represents a new conceptual approach to maintaining excellent myocardial preservation during cardiac arrest. The results also indicated that continuous cold blood cardiaplegia was better than intermittent cold blood cardiaplegia in relation to myocardial protection.
