摘要
ＤｅｔｅｃｔｉｏｎｏｆｓｏｍａｔｏｓｔａｔｉｎｍＲＮＡｉｎｒａｔｇａｓｔｒｏｅｎｔｅｒｏ－ｐａｎｃｒｅａｔｉｃｓｙｓｔｅｍｗｉｔｈｄｉｇｏｘｉｇｅｎｉｎ－ｌａｂｅｌｅｄｃＲＮＡｐｒｏｂｅｉｎｓｉｔｕｈｙｂｒｉｄｉｚａｔｉｏｎＷａｎｇＲｕｉ＇ａｎ（王瑞...
Objective: To establish a sensitive, nonradioactive in situ hybridization method to detect the expression of gut regulatory peptide genes. Methods: The digoxigenin (Dig) labeled somatostatin (SS) RNA probes were synthesized with in vitro transcription system and the in situ hybridization was conducted on the cryostat sections of rat stomach , duodenum and pancreas. Results: Positive reaction sites were purple-blue in color and located in the cytoplasm with a light background and a strong contrast. Both the morphosis and distribution of the SS mRNA posrtive cells corresponded well with those of the previously reported SS immunoreactive cells. Conclusion: The Dig -labeled cRNA probe in situ hybridization method is simple, sensitive and reliable for the study of gut regulatory peptide gene expression.
