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Effect of cytokines on the efficiency of gene transfer into murinehematopoietic progenitors

Effect of cytokines on the efficiency of gene transfer into murine hematopoietic progenitors
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摘要 The purpose of this study was to evaluate the effect of cytokines on the efficiency of gene transfer into murine hematopoietic progenitors and human K562 cells mediated by retrovirus vectors (RV) containing bacterial neomycin-resistant (neoR) gene. The bone marrow cells were preincubated with cytokines and then transfected with supernatant containing retrovirus vectors, each for 24 h. The transfected cells were plated in the semisolid culture with or without G418. The efficiency of gene transfer into hematopoietic progenitors was estimated by biological assay and PCR analysis. The most efficient combination of the cytokines, IL-1α/IL-3/SCF,increased the efficiency of gene transfer into murine CFUGM from 6.04±1. 34% to 43. 60±5. 94%. SCF alone most efficiently facilitated the gene transfer into K562 cells from 19.04±1. 58% to 54.46±2. 13%. The results suggest that the combination of IL-1α/IL-3/SCF can increase efficiency of gene transfer into hematopoietic stem cells (HSC) and progenitors, and in the treatment of acute myeloid leukemia (AML) using autologous bone marrow transplantion (ABMT),SCF can facilitate gene transfer into hematopoietic cells in gene-marking clinical studies. Effectofcytokinesontheefficiencyofgenetransferintomurinehematopoieticprogenitors¥(杨建民)(宋献民)(卢大儒)(闵碧荷)(李川晟)(孟沛霖)YangJianmin;So...
出处 《Journal of Medical Colleges of PLA(China)》 CAS 1996年第4期235-240,共6页 中国人民解放军军医大学学报(英文版)
关键词 RETROVIRUS vector neoR GENE cytokine GENE TRANSFER EFFICIENCY HEMATOPOIETIC PROGENITOR mice retrovirus vector neoR gene cytokine gene transfer efficiency hematopoietic progenitor mice
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