摘要
Ｔｈｅｒｅｌａｔｉｏｎｓｈｉｐｏｆｃａｎｃｅｒｓｕｐｐｒｅｓｓｏｒｇｅｎｅｐ５３ｗｉｔｈＨＢＶｉｎｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏｍａＷａｎｇＤｏｎｇ（王东），ＺｈａｎｇＸｉｎｌｉ（张新立），ＳｈｉＪｉｎｇｈｑｕａｎ（史景泉）（Ｄｅｐａｒｔ...
The presence of the mutations of the cancer suppressor gene p53 in hepatocellular carcinoma(HCC) appears to vary with geographic locations. Hepatitis B virus (HBV) DNA and p53 mutations were detected respectively in 38 surgical specimens of HCC in Chongqing area (where high prevalence of HCC and moderate exposure to dietary aflatoxin are known) with in situ hybridization using digoxigenin-labelled probe , PCR-restriction endonuclease cleavage and PCR-SSCP. Meanwhile , RFLP was determined in 12 specimens of the 38 with Southern blot.It was found that 79%(30/38) of the cases were positive for HBV DNA. p53 mutations were in 9 specimens in which 8 cases were positive for HBV DNA (88. 9% and P>0.05). Loss of heterozygosity was demonstrated in 2 cases out of 12 specimens of HCC(16.7%) and both the 2 cases were positive for HBV DNA 20% and P>0.05).These findings suggest that there is a significant correlation between HBV infection and HCC in Chongqing area and HBV may act synergistically with aflatoxin to induce p53 mutations and subsequently the development of HCC.
