摘要
Ｅｆｆｅｃｔｓｏｆｅｓｔｒｏｇｅｎｏｎｇｒｏｗｔｈｏｆｂｒｅａｓｔｃａｎｃｅｒｃｅｌｌｓａｎｄｅｘｐｒｅｓｓｉｏｎｏｆｐ１８５ａｎｄＥＧＦｒｅｃｅｐｔｏｒＨａｏＸｉｎｂａｏ；ＺｈａｎｇＹｉｎｇｈｕａ；ＱｉｕＦｕｈａｉ；ＹｉｎＹｉｎｇ；ＷａｎｇＬｉｌｉ...
In order to study the mechanism of estrogen and other events involved in the development of breast cancer,we observed the cell growth as well as the expression of p185 and epidermal growth factor(EGF) receptor in human breast cancer cell line SK-BR-3 in the presence of estradiol(E2) using flow cytometry. The results showed that E2 could act to expedite the proliferation of breast cancer cells and promote DNA synthesis , and that the percentage in S phase rose significantly (E2 group 26. 7?2. 5,control group 21. 2?2.1, P<0. 05). Under E2 action,p185 presented a reduced expression (E2 35?5. 6, control 61?13.1, P<0. 05) while the EGF receptor expression was greatly enhanced (E, 39?6. 9, control 21?5. 4,P<0. 05),suggesting that EGF may play a more important role in early development of breast cancer.
