摘要
AIMS To screen the most potent enzyme-inhibiting Chinese medicine and observe the pharmacologic effects of the most excellent circulation-improv- ing Chinese medicine on intestinal and pancreatic hemodynamics. METHODS Screening in Vitro for amylase-and lipase-inhibiting effects of 9 Chinese traditional medicines were used. Each extract was prepared ac- cording to the method described by LIN Qi-Shou. Tests for inhibitary ef- fects on trypsin and elastase activity were carried out by following ZHANG Tian-Min's and JIANG Chuan-Kui's method. Modified Leslie's method was used for observing effect of the most excellent circulation-improving Chi- nese herb,Tao-Ren (Semen Persicae) and its extract (HHI-I),on intesti- nal hymodynamics,i.e. using electric magnetic flowmeter for intestinal bleed flow and using blood oxygenmeter for oxygen consumption. In the further experiment,laser Doppler microcirculation dynamic analyzer was used for the measurement of effect of HHI-I on pancreatic microcirculation and tissue oxygenmeter was applied for changes of pancreatic oxygen par- tial pressure. RESULTS Of the 9 screened Chinese traditional medicines,Yuan-Hu (Rhizome Corydalis) had the most potent inhibitory action on both amylase and lipase activities,followed by Da Huang,Zhi Zi and Huang Qin,Hang Shao. Canine experiments showed HHI-I could improve intestinal hymodynamics. Twenty minutes after infusion of HHI-I,intestinal blood flow increased to 225.0±68.51 ml/min,which was significantly different from that before infusion (201.34±70.21 ml/min) (P<0.05). FUrther increase was observed 40 and 60 minutes after administration (245.40± 82. 78 ml/min,P<0. 05 and 252. 20±82. 41 ml/min,P<0.01). Intesti- nal oxygen consumption also increased markedly to 5.33±2.57 ml/min 60 minutes later,significantly differing from that at zero time (2.72±1.09 ml/min,P<0.05),HHI-I could also improve pancreatic microcirculation and tissue oxygen partial pressure. Compared with the basal value (20.4 ±5.0 ml/min/100 g body wt),microcirculatory blood flow increased to 49.0±9.0 ml/min/100 g body wt 20 minutes after administration (P< 0.05). Subsequent increase was obtained 40 and 60 minutes later,reach- ing to 51.0±7.97 ml/min/100 g body wt (P<0.01) and 54.8± 15.51 ml/min/100 g body wt (P<0.01) respectively. The primary value of pancreatic oxygen partial pressure was 6.21±0.94 kPa,which reached to 7.55±1.40kPa (P<0.01),7.65±1.76 kPa (P<0.05) and 7.67±1.64 kPa(P<0.01) 20,40 and 60 minutes after HHI-I administra- tion respectively. In control group,saline had no significant effect on any indices observed above. CONCLUSIONS Yuan-Hu and Tao-Ren used together might become the new efficient medicine for treating severe acute pancreatitis (SAP).
目的筛选最强抑酶中药并观察最佳活血中药对肠及胰腺血液动力学的影响.方法应用体外筛选法观察了九味中药对胰蛋白酶及弹性蛋白酶的抑制作用,各中药提取液按林启寿法进行制备,对胰蛋白酶及弹性蛋白酶活性的抑制作用分别据张天民及蒋传葵法进行,活血化瘀最佳中药,桃仁,提取液(HHI-I),对肠血液动力学的影响依 Leslie 法实验,即电磁量计法测肠血流,应用血氧计测定肠氧耗,进一步的实验中以激光多普勒微循环动态分析仪测定了HHI-I 对胰组织微循环的影响;并以组织测氧仪观察了胰组织氧分压变化.结果九味筛选的中药中元胡(Rhizoma Coridalis)对胰蛋白酶及弹性蛋白酶的抑制作用最强,其次分别是大黄、栀子和黄芩、杭芍.犬动物实验结果表明 HHI-I 能改善肠血液动力学,给药后20 min 肠血流增至225.0±68.51 ml/min,与给药前(201.34±70.21 ml/min)比较差别显著(P<0.05);给药后40及60 min 进一步增加,分别为245.40±82.78(P<0.05)和252.20±82.4l ml/min(P<0.01).给药后肠氧耗也明显增加,60 min 时达5.33±2.57 ml/min,与给药前(2.72±1.09 ml/min)比较差别显著(P<0.05)HHI-I 还明显改善胰腺微循环及组织氧分压,微循环血流基础值为20.4±5.0 ml/min/100g,给药后20 min增至49.0±9.0 ml/min/100 g,与基础值比较 P<0.05.给药后40及60 min 增加更明显,分别为51.0+7.97(P<0.01)和54.8±15.51 ml/min/100 g(P<0.01).胰组织氧分压基础值为6.21±0.94 kPa,给药后20、40及60 min 分别增至7.55±1.40 kPa(P<0.01)、7.65±1.76 kPa(P<0.05)和7.67±1.64 kPa(P<0.01).生理盐水(对照组)对肠血液动力学及胰腺微循环和胰组织氧分压等无明显影响.结论元胡和桃仁联合应用可能成为治疗重型胰腺炎的新药.
