摘要
AIMS To study the quantitative ultrastucture of neu- roendocrine cells of gastric mucosa on normal anc pathological conditions including the duodenal ulcer (DU) and Zollinger-Ellison syndrome (ZES). METHODS The neuroendocrine cells of the gastric mucosa of eight normal subjects,six patients with DU and five patients with ZES were quantitatively investi- gated with electro microscope and ultrastructure image analyzer. RESULTS The volume density of neuroendocrine cells in DU was 1.3% and 0.8% (vs 1.6% and 0.9%,P>0.05) in gastric antrum and corpus respectively. In antrum,G cells was of 65% (P< 0.05),D cells decreased in cell density (3% vs 9.5%) and in number of cell per unit area (P<0.01). In corpus,the cell density of ECL cells increased (49% vs 30%,P<0.05);D cells and EC cells decreasec (2% P<0.01 and 4% P<0.05,respectively),and the number of D cell per unit area markedly decreased. In ZES,D cells in corpus decreased in cell density (4% vs 22%,P<0.01) and P cells also decreased (11% vs 24%,P<0.05). The density of ECL cells increased (65% vs 30%,P<0.01). CONCLUSIONS In DU and ZES,both the number and type of NE cells present some changes. Incresed gastrin in DU and ZES patients may be caused by the decrease of D cells and somatostatin secretion.
目的研究正常及病理状态包括十二指肠溃疡(DU)及 Zollinger-Ellison 综合征(ZES)胃粘膜神经内分泌细胞(NE)的超微定量分析.方法 8例正常标本,6例 DU 及5例 ZES 标本.用透射电镜及超微定量分析仪对其胃粘膜的神经内分泌细胞的各类超微定量参数进行测量.结果正常胃窦及胃体 NE 细胞密度分别为1.6%及0.9%,DU 为1.3%及0.8%(P>0.05).DU 胃窦 G 细胞占65%(P>0.05),D 细胞减少(占3%,P<0.01).胃体 ECL 细胞增多(占49%,P<0.05);D 细胞减少(占2%,P<0.01),EC 细胞减少(占4%.P<0.05),D 细胞的单位计数明显下降(P<0.01);胞浆比例明显增大(P<0.01).ZES 胃体 NE 细胞密度明显增高(为3.2%,P<0.01).其中 ECL 细胞明显增多(占65%,P<0.01);D 细胞明显减少(占4%,P<0.01).结论在 DU 及 ZES 中,NE 细胞的数量及种类都发生了变化,其中 D 细胞的减少及生长抑素的分泌下降可能是导致胃泌素升高的原因.
