摘要
Fura-2 was first used to monitor intracellular RE(3+). The characterizations of RE3+-Fura-2 interactions in solutions simulating intracellular ionic composition show that La3+ and Y3+ both form 1:1 RE(3+)-fura-2 complexes. The apparent dissociation constants are 1.61×10(-7)M,and 4.04×10(-7)M,respectively. The mouse myeloma(SP2/0) cells exposed to free RE(3+) or free RE(3+) and some small molecules in body were Studied by single cell canon measurement system.The results support the assumption that RE(3+) ions cannot enter the cytoplasm of living cells, and the small molecules used in our experiments couldn't improve the permeability of RE(3+)ions.
Fura-2 was first used to monitor intracellular RE(3+). The characterizations of RE3+-Fura-2 interactions in solutions simulating intracellular ionic composition show that La3+ and Y3+ both form 1:1 RE(3+)-fura-2 complexes. The apparent dissociation constants are 1.61×10(-7)M,and 4.04×10(-7)M,respectively. The mouse myeloma(SP2/0) cells exposed to free RE(3+) or free RE(3+) and some small molecules in body were Studied by single cell canon measurement system.The results support the assumption that RE(3+) ions cannot enter the cytoplasm of living cells, and the small molecules used in our experiments couldn't improve the permeability of RE(3+)ions.
