STUDIES ON COUNTERACTING CHROMATOGRAPHIC ELECTROPHORESIS AND ITS UTILIZATION FOR PROTEIN PURIFICATION

A counteracting chromatographic electrophoresis(CACE)separation device has been success-fully built and tested.Experiments are performed with protein mixtures of BSA,Hg,Mg and Cyto-cto study the separation performances,concentration profiles,start-up methodology as well as influ-ences of pH and ionic strength on the separation process.Mathematical models based on the fluiddynamics of the porous medium are proposed.Experiments indicate that the CACE process has agood commercial potential in protein engineering and fine chemicals purification.

A counteracting chromatographic electrophoresis (CAGE) separation device has been successfully built and tested. Experiments are performed with protein mixtures of BSA, Hg, Mg and Cyto-c to study the separation performances, concentration profiles, start-up methodology as well as influences of pH and ionic strength on the separation process. Mathematical models based on the fluid dynamics of the porous medium are proposed. Experiments indicate that the CACE process has a good commercial potential in protein engineering and fine chemicals purification.