摘要
以苹果砧木八楞海棠（Ｍａｉｎｓｍｉｃｒｏｍａｌｕｓ）为材料，对体细胞无性系的再生途径进行研究。结果表明细胞分裂素ＢＡ（１．０ｍｇ／１）对茎尖的初始分化有促进作用。在丛芽的继代培养过程中应对ＢＡ浓度进行调整，以０．５ｍｇ／１为宜，ＢＡ２．０ｍｇ／１与ＮＡＡ０．２ｍｇ／１的激素配比，可诱导叶柄组织分化不定芽，ＫＴ０．１ｍｇ／１，ＮＡＡ２．０ｍｇ／１及２．４—ＤＩ．０ｍｇ／１的激素配比可诱导无芽茎段脱分化，ＢＡＩ１．０ｍｇ／１，ＮＡＡ０．２ｍｇ／１及ＰＨ７．０可诱导愈伤组织分化成不定芽。
Three different ways of somaclone formation were established in Mains micromalus. Our results showed that the best medium for induction of initial differentiation of shoots was MS +BA 1. 0mg/1+IBA 0. 2mg/l. The optimum concentration of hormorn was BA 0. 5mg/l for subculture of cluster-buds, for differentiation of leaf stalk was BA 2. 0mg/l with NAA 0. 2mg/1,whilethat for callus formation was KT 0. 1mg/1 +NAA 2. 0mg/1+ 2. 4-D 1. 0mg/1. Adventitious budsformed from calli, cultured on the MS medium added with BA 1. omg/l+NAA 2. omg/l (pH￣ 7.0).
出处
《中央民族大学学报（自然科学版）》
1995年第1期56-58,共3页
Journal of Minzu University of China(Natural Sciences Edition)
关键词
苹果砧木
愈伤组织
分化
不定芽
rootstock
callus
differentiation
adventitious buds
