摘要
Culture of patient somatic cells, transfected with therapeutic genes into genomic DNA with retroviral vectors, followed by colony selection, amplification, and reimplantation of transformed cells into patient, has been Widely used for clinical trial of gene therapy in the past years. But the disadvantages of this protocol are obvious. (i) To a great extent the expression level of the transfected cells depends on the different integration sites,which cause the various expression rates from different colonies; besides, the life span
基金
the State High-Technology Development Program of China.
