用RAPD标记评价百合品种间遗传关系

Using Rapd Marker to Evaluate The Genetic Relationship Among Genus Lilium

本文从１３个百合品种种１野生种中分别提取了总ＤＮＡ，进行ＰＣＲ－ＲＡＰＤ分析，每个品种都得到了各自独特的带谱，通过对这些带谱的分析及数据的处理，并用计算机计算出百合品种间的遗传关系。结果表明，ＲＡＰＤ技术能用以鉴定百合品种间的遗传关系并推测其亲缘关系，本文对实验中遇到的一些问题进行了讨论。

After isolating the DNA of 13 cultivars and 1 native Chinese species fromgenus Lilium,we detected DNA polymorphism by means of RAPD. Every cultivarstudied has its own RAPD bands, and they are totally different from the others.Through the analysis of the data of the bands, we gained some information of thegenetic relationship among the cultivars. From all above, we considered that theRAPD is a powerful tool for distinguishing the cultivars of the Lilium and analysisof the genetic relationship among them.