甘蓝下胚轴离体培养直接分化成苗的研究

A Sudy on the Direct Plant Regeneration of Hypocotyl of Cabbage

经正交试验Ｌ１６（４３）分析了ＢＡ、ＩＡＡ及营养物质ＬＨ对甘蓝下胚轴分化成苗的影响。结果表明：ＢＡ对甘蓝下胚轴分化的作用最显著，ＢＡ０．５ｍｇ／Ｌ，ＩＡＡ０．０１ｍｇ／Ｌ和ＬＨ６００～８００ｍｇ时促进芽的分化，ＢＡ为０．５ｍｇ／Ｌ、ＩＡＡ０．１ｍｇ／Ｌ以及ＬＨ８００ｍｇ／Ｌ时促进下胚轴长根。甘蓝叶片，下胚轴不宜诱导愈伤组织。

We selected hypocotyl of cabbage(Brassica oleracea Var.Capitata L.) for the exepriment of tissue culture in order to study the direct regeneration of plant,By theorthogonal test, we analysed the effect of BA、IAA and LH toregeneration of hypocotyl of cabbage.The result show: BA have get main effectto regeneration.In the direct plant regeneration media with BA of 3.5mg/L,IAA of 0.01mg/L and LH of 600 800mg/L,the bud can be developed, a mediawith BA of 0.5mg/L, IAA of 0.1mg/L and LH of 600～800mg/L,the bud can be developed;a media with BA of 0.5mg/L,IAA of 0.1mg/L and LH of 800mg/L,the root be grown.The leaf and hypocotyl do not suitable induced the calls.